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. 2020 Jul 31;9(8):971. doi: 10.3390/plants9080971

Figure 6.

Figure 6

Somatic embryogenesis (SE) trials on peach mature explants. (a) In vitro meristematic bulk (MB) of “Hansen 536”; the arrow indicates the type of young leaf collected and used as starting explant in SE induction experiment (bar = 1 cm). Brownish calli (arrow) developed from “Hansen 536” leaves cultured on medium C (b) and on medium D (c); the images were taken after 3 months from the beginning of the experiment (bar = 2 mm). Cream-colored calli (arrow) developed from “Hansen 536” leaves cultured on medium A (d), on medium B (e), and on medium E (f); the images were taken after 3 months from the beginning of the experiment (bar = 2 mm). Necrotic calli from “Hansen 536” leaves cultured on plant growth regulator (PGR)-free medium (g) after 4 months from the beginning of the experiment (bar = 1 cm). Cuttings of peach rootstock “GF677” (h) and sterile unopened flowers of “GF677” (i) used as starting explants in the SE induction experiment (bar = 1 cm). Cream-colored calli (arrow) developing from petal (j) and anther with filament (k) of “GF677”, both cultured on PAM medium after approximately 3 months from the beginning of the experiment (bar = 2 mm). (l) Cream-colored calli formation (arrow) from “GF677” anther with filament cultured on PIV medium [47] (bar = 2 mm). (m) “GF677” anthers with attached filament cultured on MSI medium [52] for 3 months (bar = 1 cm).