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Gel electrophoresis analysis of in vitro ability of CRISPR pDNA delivered by alginate nanoparticles to induce double-strand breaks (DSBs) of targeted DNA. (left) PCR amplicon of GFP pDNA delivered to cells treated with Cas 9 only; (right) PCR amplicon of EGFP pDNA delivered to cell treated with (CRISPR ALG NPs). Black arrows indicate the new bands results from pDNA cut.
