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. 2020 Aug 11;12(8):2247. doi: 10.3390/cancers12082247

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression levels of AR-V7, AR-FL, and KLK3 in whole blood. Following reverse transcription and preamplification, the expression of AR-V7, AR-FL, KLK3, and β-Glucuronidase (GUSB) in whole blood of CRPC patients (n = 16) and healthy controls (n = 16) was analyzed by qPCR assays. After normalization to GUSB, the lowest normalization (ΔCq) value of the control samples was used as the threshold for positive tests, as indicated by the line. Positive tests are depicted as green data points. For AR-V7, nine control samples and nine patient samples yielded no Cq-values (45 qPCR cycles). They were assigned a Cq-value of 46, normalized to GUSB, and plotted as grey data points.