Table 1.

Cell counts of controls analyzed by in situ padlock probe technology.

Gene	Total Cell Count	CellCollector Healthy Controls				PBMC Healthy Controls				PC Cell Lines
		Ctrl 1	Ctrl 2	Ctrl 3	Ctrl 4	Ctrl 5		Ctrl 6		PC-3		VCaP
		# cells				# cells	%	# cells	%	# cells	%	# cells	%
		N.A.	N.A.	N.A.	N.A.	33,855	100.00	34,544	100.00	4573	100.00	2200	100.00
KLK3	0 RCPs/cell	N.A.	N.A.	N.A.	N.A.	33,855	100.00	34,544	100.00	4573	100.00	2181	99.14
	1 RCP/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	18	0.82
	2 RCPs/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	1	0.05
	3 RCPs/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	0	0.00
	>3 RCPs/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	0	0.00
AR-V7	0 RCPs/cell	N.A.	N.A.	N.A.	N.A.	33,593	99.23	34,298	99.29	4511	98.64	411	18.68
	1 RCP/cell	5	9	6	6	262	0.77	246	0.71	62	1.36	451	20.50
	2 RCPs/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	423	19.23
	3 RCPs/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	296	13.45
	>3 RCPs/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	619	28.14
AR-FL	0 RCPs/cell	N.A.	N.A.	N.A.	N.A.	33,486	98.91	34,213	99.04	4502	98.45	51	2.32
	1 RCP/cell	6	4	1	3	360	1.06	327	0.95	70	1.53	58	2.64
	2 RCPs/cell	0	1	0	0	9	0.03	4	0.01	1	0.02	79	3.59
	3 RCPs/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	121	5.50
	>3 RCPs/cell	0	0	0	0	0	0.00	0	0.00	0	0.00	1891	85.95

This table summarizes the number and percentage of cells with 0, 1, 2, 3, or >3 RCPs/cell for KLK3, AR-V7, and AR-FL. Shaded rows represent the cut-off used for the identification of CTCs in patient samples. Total cell number was not counted on CellCollectors (N.A. = not available). VCaP cells were used as positive control. PBMC: peripheral blood mononuclear cells; RCP: rolling circle product; PC: prostate cancer; Ctrl: control.