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. 2020 Aug 15;9(8):1905. doi: 10.3390/cells9081905

Figure 4.

Figure 4

In vivo uptake of Cy5-scsiRNA-loaded RS800-(Non-)ManNP on cellular level. Fibrotic livers were harvested 12 h after a single IV injection of Cy5-scsiRNA-loaded RS800-(Non-)ManNP (2 mg/kg siRNA). (A) In vivo cellular uptake of both carriers (RS800-NonNP and -ManNP) in (non-)parenchymal liver cells as assessed by FACS analysis of single cell suspensions obtained from harvested livers. Cell-specific uptake in relation to hepatocytes as most abundant liver cells was significantly enhanced for RS800-ManNP in M2 polarized macrophages (CD45+, F4/80+ and CD206+) after normalization to hepatocyte uptake (** p < 0.001). (B) Confocal fluorescence laser microscopy of liver cryosections obtained from fibrotic mice. Liver sections were stained for the mannose receptor CD206 (green) and Cy5-siRNA (red). Colocalization of carriers with CD206+ cells is indicated by yellowish staining. Sections of fibrotic mice injected with PBS as vehicle control served to normalize for background fluorescence (Figure S4).