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. 2020 Aug 6;9(8):1847. doi: 10.3390/cells9081847

Figure 1.

Figure 1

Expression of EpCAM and TROP2 in intestinal epithelial cells (IEC) of transgenic and control mice. (a) Absolute numbers of murine EpCAM, murine TROP2, and human EpCAM transcripts in 1 ng intestinal total RNA were determined using quantitative RT-PCR. Data represent means ± SD in samples from 4 mice in each group (n = 4). ** p < 0.01, **** p < 0.0001 using a one-way ANOVA with Tukey’s multiple comparison test. n.d. means not detected. (b) Immunofluorescence microscopy verifying expression of murine EpCAM (green) and murine TROP2 (red) in the small intestines of wild type (WT) and murine TROP2 rescue (T2R) mice. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars, 100 μm. (c) Immunofluorescence microscopy verifying expression of murine EpCAM (green) and human EpCAM (red) in the small intestines of WT and human EpCAM rescue (hEpR) mice. Nuclei were stained with DAPI (blue). Scale bars, 100 μm.