Figure 6.

Characterization of organoids and spheroids from TROP2 rescue mice and relevant controls. (a) Representative serial phase contrast photomicrographs of IEC organoids obtained from WT, Ctrl (T2), T2R, and hEpR intestinal crypts in vitro. Scale bars, 100 μm. (b) Forming and passaging efficiencies of organoids from relevant mice. Organoid-forming efficiencies represent ratios of organoid numbers per well at day 9 to crypt numbers per well at day 1 (left panel). Aggregate data (means ± SD) from four independent experiments are presented. Ten wells were examined in each experiment. Organoid passage efficiencies represent ratios of organoid numbers/well at day 9 of secondary cultures to organoid numbers/well at day 9 of primary cultures (right panel). Aggregate data (means ± SD) from four independent experiments are presented. Six wells were examined in each experiment. ** p < 0.01, **** p < 0.0001 as determined via one-way ANOVA with Tukey’s multiple comparison test. (c) Representative phase contrast photomicrographs of spheroids from WT, Ctrl(T2), T2R, and hEpR mice on day 2 after initial passage and fourth passage as indicated. Scale bars, 100 μm.