Figure 5.

Effect of IQGAP3 suppression on invasion and anchorage-independent growth of gastric cancer cells. (A) Reduced the number of migrating MKN1 (left) and TMK1 cells (right) by si-IQGAP3 compared to control siRNA (* p = 0.001 and 0.033, respectively; two-tailed Student’s t-test). Matrigel assays were carried out in triplicate. (B) Anchorage-independent growth of TMK1 cells treated with control siRNAs, or si-IQGAP3 by soft agar assay in triplicate (* p = 0.03 and 0.01, respectively; two-tailed Student’s t-test). The relative number of visible colonies (to that of TMK1 cells transfected with si-control-1) was calculated. (C) Representative pictures of anchorage-independently growing colonies from MKN1 cells in soft agar. Magnification: ×40.