Figure 9.

Experimental design to identify the toxicity determinants of Aβ₄₀ oligomers. (1) A library of Aβ₄₀ oligomers was prepared by incubating Aβ₄₀ oligomers with a collection of seven catechins expected to remodel to varying extents their pre-existing toxic conformation into a less toxic conformation. (2) The toxicity of the different oligomers was evaluated by using Presto Blue assay, which relies on mitochondrial activity to inform on cell viability, and PI fluorescence, which reports if the cell membrane is being compromised. (3) Different techniques with different degrees of resolution were implemented to extensively characterize the molecular features of the oligomers present in the Aβ-library. (4) Correlation studies between toxicity (2) and molecular features (3) were necessary to reveal the key determinants of toxicity. Figure in (4) can be found in reference [34] Chem. Sci. 2019, 10, 6072–6082. Published by The Royal Society of Chemistry.