Figure 2.

GLN impact on the expression of mRNAs encoding neurotrophins and the BDNF protein in the hippocampus, striatum and cortex. The RNAs were extracted from the hippocampus (A), striatum (B) and cortex (C), followed by the RT-PCR assay to detect the mRNAs of BDNF, NGF, NT-3, NT-4 and CNTF using GAPDH as an internal control. Protein samples were also prepared from the same tissues and the expression of BDNF and pro-BDNF was analyzed by Western blotting with β-actin as an internal control (D). The results represent the means ± S.D. (n = 8) *, p < 0.05, compared with the 0 mg group.