Figure 3.

GLN regulation of CREB5 and PDE4B mRNAs, cAMP accumulation and CREB phosphorylation in the hippocampus and the expression of BDNF, CREB5 and PDE4B in the hippocampal cells. The RNA samples prepared from GLN-injected animal’s hippocampus (A) and from GLN-treated HT22 cells (D) were subjected to RT-PCR to analyze the mRNAs of BDNF, CREB5 and PDE4B. The cAMP concentration (B) and CREB phosphorylation (C) in hippocampal tissues were determined. Protein expression of BDNF, CREB5 and PDE4B in GLN-treated HT22 cells were analyzed by Western blotting (E,F). The results represent the means ± S.D. (n = 8). *, p < 0.05 compared with the 0 mg (A–C) or the 0 mM group (D,F).