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. 2020 Aug 12;25(16):3667. doi: 10.3390/molecules25163667

Figure 4.

Figure 4

GLN impact on the cAMP/PKA signaling in relation to BDNF production in HT22 cells. HT22 cells were exposed to GLN (10 mM) for indicated times and the CREB phosphorylation manner was analyzed with Western blotting normalized by the β-actin (A). To examine the GLN effect on the CRE reporter activity, HT22 cells were cotransfected with a CRE reporter plasmid and a p-CMV-β-gal control plasmid, followed by the treatment with GLN (10 mM) alone or in combination with H89 (10 and 20 µM) for 24 h. The luciferase activity in cell lysates was analyzed and normalized against the β-gal activity within the same sample (B). To reveal the significance of the GLN-induced cAMP/PKA signaling in BDNF expression, HT22 cells were treated with GLN (10 mM) alone or in combination with H89 (20 µM) for 24 h and BDNF expression was determined (C). The results represent the means ± S.D. (n = 3–4). *, p < 0.05 compared with the 0 mM group (A–C); #, p < 0.05 compared with the control group within the same time point (A) or with the GLN alone group (B,C).