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. 2020 Sep 1;11:68. doi: 10.1186/s13229-020-00373-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Metabolism of NL4X protein. a Schematic depiction of NL4X metabolism. Positions of ASD/XLMR-associated variants are indicated by arrow. Epitopes of antibodies are also shown. b Characterization of antibodies used in this study. c Protein metabolism of overexpressed NL4X in COS-1 cells. d Deglycosylation assay of overexpressed NL4X in COS-1 cells. e Cell surface biotinylation of overexpressed NL4X in COS-1 cells. f Detection of endogenous NL4X in IMR32 cells. g Immunoprecipitation of endogenous sNL4X secreted from IMR32 cells. h Deglycosylation assay of endogenous NL4X in IMR32 cells. i Detection of endogenous NL4X in iPSC-derived human neurons. j Immunoprecipitation of endogenous sNL4X secreted from iPSC-derived human neurons. k Immunoblot analysis of the lysates of the NL4X KO iPSC-derived human neurons