Fig. 5.

Protein metabolism and synaptogenic function of NL4X Δ651-652 mutant. a Representative immunoblot of cell lysates of COS-1 cells transfected with NL4X Δ651-652 mutant. b Quantitative analysis of the western blot for mature NL4X (n = 3, **p < 0.01 vs WT by Tukey-Kramer multiple comparisons test). c Representative immunoblot of conditioned medium from COS-1 cells transfected with NL4X Δ651-652 mutant. d Quantitative analysis of the western blot for sNL4X (n = 4, **p < 0.01 vs WT, by Student t test). e Representative immunoblot of biotinylated cell surface NL4X Δ651-652 mutant. f Quantitative analysis of the western blot for biotinylated WT and variants (n = 4, **p < 0.01 vs WT by Tukey-Kramer multiple comparisons test). g Representative images of synaptogenic activity of NL4X Δ651-652 mutant in HEK293 cells. Scale bar 10 μm. h Quantitative result of synaptogenic activity of NL4X Δ651-652 mutant (n = 4–6, *p < 0.05, **p < 0.01 vs WT by Tukey-Kramer multiple comparisons test)