Table 3.
Pathogen | Test procedure (positive titre) | Number positive/number tested |
---|---|---|
Bluetongue virus | AGID (1:4) | 0/29 |
Infectious bovine rhinotracheitis (Bovine herpesvirus) | ELISA | 0/29 |
Bovine viral diarrhoea virus | ELISA | 0/29 |
Brucellosis | BPA /ROSEBEN /2ME (1:100) / SAT (1:100) | 1/29 |
Foot-and-mouth disease virus | VIAA | 0/29 |
Johne’s disease (Mycobacterium avium subsp. paratuberculosis) | AGID | 0/29 |
Leptospira interrogans (11 serovars) a | MAT (1:50) | 2/29 |
Bovine leucosis | AGID | 0/29 |
Q Fever b | Indirect multi-species ELISA | 0/29 |
Chlamydial abortion c | Indirect multi-species ELISA | 0/29 |
Vesicular Stomatitis Virus Indiana and New Jersey Serotype | ELISA-LP (liquid phase) | 0/29 |
References: AGID: agar gel Immunodiffusion; ELISA: enzyme-linked immunodiffusion assay; BPA: buffered plate antigen test; ROSEBEN: rose bengal test; 2ME: 2-mercaptoethanol test; SAT: tube agglutination test; VIAA: virus infection-associated antigen; MAT: microagglutination test. Tests performed at National Service of Agri-food health and quality (SENASA)
aLeptospira interrogans serovars ballum, castellonis, canicola, grippotyphosa, icterohaemorrhagiae, copenhageni, pomona, pyrogenes, sejroe, wolffi, tarassovi
bID Screen® Q Fever Indirect ELISA Multi-species kit (ID.vet, France)
cID Screen® Chlamydophila abortus Indirect ELISA Multi-species kit (ID.vet, France)