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. 2020 Aug 13;8(8):1235. doi: 10.3390/microorganisms8081235

Figure 1.

Figure 1

Analysis of purified recombinant Com1 protein with His-tag. Ni-NTA purified recombinant Com1 protein (predicted molecular weight 29.9 kDa, 30 µg per lane) was separated using a 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (a) For Western blot analysis mouse anti-6xHis monoclonal antibody (1:5000, Clontech Laboratories, Inc., Mountain View, CA, USA) was used and traced with alkaline phosphatase labeled goat anti-mouse secondary antibody (1:5000, Sigma-Aldrich, St. Louis, MO, USA). (c) Recombinant Com1 protein was visualized using colloidal Coomassie Blue. Lane (a,c), purified recombinant Com1 protein; Lane (b), protein marker with size in kDa (Thermo Fisher Scientific, Waltham, MA, USA).