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Cloned and expressed Com1 protein. (a) Insertion of com1 into pET300/NT-DEST vector for expression of recombinant Com1 of 29.9 kDa with His-tag. (b) Expression of His-tagged com1 as recombinant Com1 protein. ATG, vector encoded start codon; RBS, vector encoded ribosome binding site; 6xHis, vector encoded His-tag. Axis counting base pairs in relation to com1 Figure A2.
