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. 2020 Jul 30;11(8):866. doi: 10.3390/genes11080866

Figure 2.

Figure 2

Displacement of 8-oxoguanine–DNA glycosylase (OGG1) by DNA polymerase β. (A) POLβ interacting with OGG1 bound to the template strand; (B) POLβ interacting with OGG1 bound to the downstream strand. White circles show the secondary substrate cleavage in the absence of POLβ, black circles, in the presence of POLβ and dNTPs and black triangles, in the presence of POLβ without dNTPs. Mean ± SEM of 3–5 independent experiments are presented; (C) a representative gel of the cleavage experiment. Lanes 1–5, both POLβ and dNTPs are present; lanes 6–10, no POLβ; lanes 11–15, POLβ without dNTPs. OGG1 was on the downstream strand. The arrows mark the mobility of the substrate (S) and β-elimination product (P). The incubation time is indicated above the gel; (D) cleavage of the secondary substrate by OGG1 with or without POLβ in the absence of the primary substrate; (E) a representative gel of the primer extension experiment. The substrate and the conditions were the same as in Panel C. The arrow marks the mobility of the primer.