Figure 5.

Time course of Schiff base decay in the reaction catalyzed by OGG1 in the presence and in the absence of DNA polymerases. (A) POLβ interacting with OGG1 bound to the template strand; (B) POLβ interacting with OGG1 bound to the downstream strand; (C) KF interacting with OGG1 bound to the template strand; (D) KF interacting with OGG1 bound to the downstream strand. White circles show the secondary substrate cleavage in the absence of a DNA polymerase, black circles, in the presence of a polymerase and dNTPs and black triangles, in the presence of a polymerase without dNTPs. Mean ± SEM of three independent experiments are presented.