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. 2020 Aug 6;25(16):3576. doi: 10.3390/molecules25163576

Table 7.

Sequences of CatS1–2 and CatK1–2 and their reported biological properties [99].

Comp. Sequence a Ki for CatS, pH 5.5 [nM] b Ki for CatS, pH 7.4 [nM] b Ki for CatK, pH 5.5 [nM] b Ki for CatL, pH 5.5 [nM] b
CatS1 Ac-Gly-Arg-Trp-His-Pro-Met-GlyΨ[Tz]Ala-Pro-Trp-Glu-D-Ala-D-Arg-NH2 15,000 ± 5000 42,000 ± 8000 10,000 ± 3600 30,000 ± 3200
CatS2 Ac-Gly-Arg-Trp-His-Pro-Met-azaGly-Ala-Pro-Trp-Glu-D-Ala-D-Arg-NH2 26 ± 5 17 ± 3 3 ± 0.8 5 ± 0.5
CatK1 Abz-Arg-Pro-Pro-GlyΨ[Tz]Phe-Ser-Pro-Phe-Arg-Tyr(3-NO2)-NH2 n.o. n.o. 800 ± 330 22,000 ± 2000
CatK2 Abz-Arg-Pro-Pro-azaGly-Phe-Ser-Pro-Phe-Arg-Tyr(3-NO2)-NH2 n.o. n.o. 9 ± 0.7 2000 ± 400

a Abz: o-Aminobenzoic acid b Ki values were determined by measuring residual peptidase activity after incubation with the peptidomimetics at 37 °C using Z-Leu-Arg-AMC (Cathepsin S) or Z-Phe-Arg-AMC (Cathepsin K and L) as substrate. Data expressed as the means ± SEM of three individual experiments. n.o.: not observed.