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1H-15N HSQC NMR spectra of TgCEN1-C and TgCEN2-C. (A) NMR spectra of apo- or Ca2+-bound 15N-labelled TgCEN1-C or TgCEN2-C. The apo-condition was obtained by adding 5 mM EGTA, while the Ca2+-bound condition was obtained by adding 5 mM CaCl2. (B) Downfield spectral region of 1H-15N HSQC spectra showing Gly-6 peaks of TgCEN1-C and TgCEN2-C variants in the presence of 5 mM CaCl2.
