Figure 7.

Turbidity measurements of full-length and truncated TgCEN1 (A) and full-length and truncated TgCEN2 (B). Scattering intensity at 340 nm was measured as a function of time at 37 °C for the samples (20 μM protein) with 1 mM Ca²⁺ or 1 mM EGTA. (A) Representative association profiles of Ca²⁺-bound full-length TgCEN1 (black), apo-full-length TgCEN1 (red), Ca²⁺-bound TgCEN1-C (blue) and apo-TgCEN1-C (green). The effect of EGTA on a solution of Ca²⁺-saturated full-length TgCEN1 is also shown (light violet). (B) Representative association profiles of Ca²⁺-bound full-length TgCEN2 (black), apo-full-length TgCEN2 (red), Ca²⁺-bound TgCEN2-C (blue) and apo-TgCEN2-C (green).