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Thermodynamic parameters for Ca2+ binding to TgCEN1-C and TgCEN2-C. The mean values ± SEM from triplicate experiments using at least two different protein preparations are presented.
| Binding Sites Used for Modelling |
n | Ka (M−1) |
ΔH (kcal mol−1) |
|
|---|---|---|---|---|
| TgCEN1-C | 2 | n1 = 0.7 ± 0.1 | 4.8 × 105 ± 6.1 × 103 | −9.6 ± 1.1 |
| n2 = 1.2 ± 0.3 | 3.9 × 104 ± 4.5 × 103 | −3.2 ± 0.7 | ||
| TgCEN2-C | 1 | 0.8 ± 0.1 | 1.6 × 104 ± 1.5 × 103 | −3.4 ± 0.4 |
