Figure 2.

HPLC (high pressure liquid chromatography) based quantification of polyamines (PA) in heat-stressed tissues. (A) Free PAs—PUT, SPD, and SPM. (B) Conjugated PAs—PUT, SPD, and SPM. (C) Bound PAs—PUT, SPD, and SPM. A minimum of three leaves were excised from three plants for each biological replicate. Mean data points and standard error of a minimum of three biological replicates are shown. Statistical significance (p-value) was derived using Graph prism pad program via Tukey test/t-test.