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. 2020 Jul 22;9(8):1749. doi: 10.3390/cells9081749

Figure 4.

Figure 4

HPLC (High pressure liquid chromatography) based quantification of polyamines (PA) levels in cold-stressed tissues. (A) Free PAs—PUT, SPD, and SPM. (B) Conjugated PAs—PUT, SPD, and SPM. (C) Bound PAs—PUT, SPD, and SPM. A minimum of three leaves were excised from three plants for each biological replicate. Mean data points and standard error of a minimum of three biological replicates are shown. Statistical significance (p-value) was derived using Graph prism pad program via Tukey test/t-test.