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. 2020 Jul 22;9(8):1749. doi: 10.3390/cells9081749

Figure 5.

Figure 5

Cold-induced expression of PA metabolic pathway genes in tomato plants exposed to 4 °C temperature. (A–D) qRT-PCR led relative gene expression analysis of tomato leaf tissue reveals differential abundance of putrescine (PUT) biosynthesis genes (SlARG1, 2, SlADC1, 2 SlODC1, 2, SlAIH and SlCPA); (E–G) Spermidine (SPD) and Spermine (SPM) biosynthesis genes (SlSAMDc1, 2, 3, SlSPDS1, 2 and SlSPMS); (H,I) PA catabolic pathway genes (SlPAO2 and SlPAO4-Like; SlCuAO and SlCuAO4-like); (J–L) Cold-responsive genes CBF1, 2 and 3. A minimum of three leaves were excised from three plants for each biological replicate. Mean data points and standard error of a minimum of three biological replicates are shown. Statistical significance (p-value) was derived using Graph prism pad program via Tukey test/t-test. SlUBI3 and SlTIP41 were used as reference genes.