Skip to main content
. 2020 Aug 6;9(8):1843. doi: 10.3390/cells9081843

Figure 2.

Figure 2

(A) Mitochondrial membrane potential (ΔΨm) of intact cells measured using red/green fluorescence distribution of the ratiometric JC-1 probe (n = 6). (B) Mitochondrial reactive oxygen species (ROS) production in intact cells measured as % of MitoSOX fluorescence (n = 4). (C) 4-hydroxynonenal (4-HNE), a product of lipid peroxidation, immunoblotted on cells stably expressing EV and WT, S47A, S47E Cytc. (D) Quantification of 4-HNE band density using ImageJ software (n = 3). (E) ATP levels in the cell lines stably expressing EV and WT, S47A, S47E Cytc (n = 3). Data are represented as means ± SEM, * p < 0.05.