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. 2020 Aug 1;9(8):200. doi: 10.3390/biology9080200

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Shoaling endpoint comparisons between the AB strain zebrafish (white), absolute zebrafish (red), TL (Tübingen Long Fin) zebrafish (blue), golden zebrafish (green), PET (Pet Store-purchased) zebrafish (purple), and the WIK (Wild Indian Karyotype) zebrafish (orange). (A) Average inter-fish distance, (B) average shoal area, (C) average nearest neighbor distance, and (D) the average furthest neighbor distance were analyzed for the shoaling assay. Groups of three fish were tested for shoaling behavior. The median and the interquartile for the violin plot were labeled with the bold line colored with cyan and yellow, respectively. The 5 min locomotor trajectories for the AB, PET, and WIK zebrafish are presented in (E–G), respectively. The faint grey line indicates the borderline between the top and bottom portions of the test tank. The data were analyzed by a Mann–Whitney test (n = 30 for each group, except for the WIK zebrafish (n = 21); * p < 0.05; *** p < 0.001).