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Effect of fisetin, 5-FU or their combination on apoptosis by annexin-V-FLUOS staining in (a) HCT116 cells and (b) HT29 cells. The cells were treated with fisetin (30–90 μM), 5-FU (50 μM) or their combination and resuspended in Annexin-V-FLUOS labeling solution. The data was collected on a Becton-Dickinson FACSCalibur (San Jose, CA) and analyzed in FlowJo, Version 9.7 (FLowJo, LLC, Ashland, OR). [Color figure can be viewed at wileyonlinelibrary.com]
