Figure 5.

Effect of the treatment of fisetin, 5-FU or their combination on colorectal tumorigenesis in FC¹3K¹Apc^Min/+ mice. (a) Effect of the treatment of fisetin, 5-FU or their combination on colon tumor incidence in FC¹3K¹Apc^Min/+ mice that had no visible tumors at the beginning of treatment. (b) Effect of the treatment of fisetin, 5-FU or their combination on tumor growth (early) in FC¹3K¹Apc^Min/+ mice. The size of each tumor at each colonoscopy visit was calculated by determining what percentage of the fully inflated lumen was occluded by the tumor and plotted with each different colored line representing a distinct tumor. (c) The calculation was made from still images of each tumor that were collected during each colonoscopy visit. NA, not available, because the control became moribund and was euthanized prior to the fourth colonoscopy visit. (d) Effect of the treatment of fisetin, 5-FU or their combination on tumor multiplicity in FC¹3K¹Apc^Min/+ mice. When mice became moribund or the study ended, experimental mice in all groups were euthanized and necropsied to score the number of intestinal tumors. The small intestine was divided into four equal segments with S1 being closest to the stomach and S4 being closest to the cecum; S1 was combined with S2 and S3 was combined with S4 because the FC transgene is expressed only in S3, S4 and the colon. [Color figure can be viewed at wileyonlinelibrary.com]