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. 2020 Aug 18;9(8):1917. doi: 10.3390/cells9081917

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Dot-blot analysis of the efficacy of TPO(+)EV isolation. (A) Blots with similar samples (upper line: Total EV population; middle line: Supernatant TPO(+)EV-depleted; bottom line: SPMP-TPO(+)EV complexes) stained with antibodies against TPO and CD9. Blots included samples from 5 patients. (B) The result of 10 samples for dot-blotting were quantified using ImageJ. The ratios of TPO/CD9 signal intensities were calculated and averaged in three groups, presenting SPMP-TPO(+)EV complexes, supernatant TPO(+)EV-depleted, and the total EV population.