Figure 4.

Promotion of Ca wave generation by Fe and attenuation by mitochondrial permeability transition pore (mPTP) Inhibition. (A) A representative Ca fluorescence trace showing the effect of Fe³⁺/HQ-8 (5, 15, and 100 µM) on the frequency of spontaneous Ca wave formation in a WT ventricular myocyte. (B) Summarized data obtained from 10 WT myocytes. (C,D) The same as (A,B), except in CypD KO ventricular myocytes (n = 9). (E,F) The same as (A,B), except that the WT myocytes were pretreated with 1 µM CsA before Fe perfusion (n = 7). * p < 0.05, ** p < 0.01, compared to respective control value by paired t-test.