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. 2020 Jul 28;12(8):2097. doi: 10.3390/cancers12082097

Figure 2.

Figure 2

Effects of OME and esomeprazole (ESO) on AhR-dependent invasion and gene expression in wild-type 15-037 siAhR cells. Wild-type 15-037 and AhR-deficient 15-037 cells were treated with DMSO, 100 µM OME, and 100 µM ESO and spheroid cell invasion was determined and quantitated (A). AhR expression after knockdown by siAhR in 15-037 cells was determined by Western blot of whole cell lysates (B). Effects of OME and ESO on CYP1A1 expression in wild-type and AhR-deficient 15-037 cells (C), effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), OME, and ESO in CYP1A1 induction in established and patient-derived GBM cells (D), and effects of OME and ESO on CXCL12, CXCR4, and MMP9 expression in wild-type and AhR-deficient 15-037 cells (E) was determined by real time PCR as outlined in the Materials and Methods. Results (A,C–E) are means ± SD for at least 3 determinations for each treatment group and significant (p < 0.05) effects by the compounds alone (*) and attenuation of these responses after AhR knockdown (**) are indicated.