Skip to main content
. 2020 Jul 28;12(8):2097. doi: 10.3390/cancers12082097

Figure 3.

Figure 3

Effects of OME and ESO on AhR-dependent invasion and gene expression in 15-037 and 15-037 AKO-19 cells. Wild-type 15-037 and 15-037 AKO-19 cells were treated with DMSO, 100 µM OME, and 100 µM ESO and spheroid cell invasion was determined and quantitated (A) as outlined in the Materials and Methods. AhR expression in 15-037/15-037 AKO-19 cells were determined by Western blots of whole cell lysates (B). Effects of DMSO (set at 1.0), 100 µM OME, and 100 µM ESO on gene expression in 15-037 and 15-037 AKO-19 cells (C) were determined by real time PCR as outlined in the Materials and Methods. Results (A,C) are expressed as means ± SD for at least 3 determination for each treatment group and significant (p < 0.05) effects by the compounds alone (*) and attenuation of these response after silencing AhR (**) are indicated.