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. 2020 Aug 18;12(8):2331. doi: 10.3390/cancers12082331

Figure 3.

Figure 3

P. gingivalis intracellular survival in PDAC cells can be enhanced in vitro. (a,b) Intracellular P. gingivalis CFU recovered 48 h and 72 h after infection. (c,d) Intracellular “original” vs. “enhanced” P. gingivalis (see text for explanation) recovered immediately after antibiotic treatment (0 h) or after 72 h in normoxic or hypoxic conditions. Experiment was performed three times. Data show the mean ± SD of a representative experiment (n = 2 wells per condition). Two-way ANOVA was performed for statistical analysis. *** p ≤ 0.005. (e,f) FITC-labeled P. gingivalis was used to infect PANC1 or Mia Paca-2 cells. After 24 h infection, cells were fixed and nuclei were stained with DAPI prior to visualization using a NIKON confocal microscope at 60× magnification. One cell is magnified and shown with brightfield overlay for each image. The experiment was performed twice.