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Myogenic nature of iHMuSC clones. (A,B) Primary HMuSCs and iHMuSC clones were tested for CDK4 (A) and TERT (B) gene expression using RT-qPCR. (C) Immunostaining for the myogenic markers Pax7 (Red) and desmin (green) in growing iHMuSC clones. (D) Immunostaining desmin (green) and dystrophin (red) in differentiated iHMuSC clones. Nuclei are labelled with Hoechst (blue). Bars = 100 µm.
