Table 1.
Primer Name | Sequence | Anneling Temperature |
---|---|---|
mCherry NheI Forward | GAGATCGCTAGCGGGCCCGCCACCATGGTGAGCAAGGGCGAGGAG | 60.9 °C |
mCherry XhoI CMV Reverse | GAGATCCTCGAGCTACTTGTACAGCTCGTCCATG | 60.9 °C |
Promoter EF1ɑ Forward | TCGGGTTTTTCGAACGGCTCCGGTGCCCGTCAG | 60 °C |
Promoter EF1ɑ Reverse | ATGGTGGCGGGCCCGTCACGACACCTGAAATGGAAG | 60 °C |
Promoter PGK/UBC Forward | GATCTCGACGGTATCGAAAGC | 60 °C |
Promoter PGK Reverse | CCATGGTGGCGGGCCCGAATTCGATCTCGGATCCGAAAGCGAAGGAGCAAAGCTG | 60 °C |
Promoter UbC Reverse | CCATGGTGGCGGGCCCGAATTCGATCTCGGATCCGTCTAACAAAAAAGCCAAAAAC | 60 °C |
AP3D1 Forward | GGCATCCGTAACCACAAGGA | 60 °C |
AP3D1 Reverse | TTGTCCTGCTTCAGCTCCTG | 60 °C |
CDK4 Forward | GGCTGAAATTGGTGTCGGTG | 60 °C |
CDK4 Reverse | CACGAACTGTGCTGATGGGA | 60 °C |
TERT Forward | TGTACTTTGTCAAGGTGGATGTGA | 60 °C |
TERT Reverse | GCTGGAGGTCTGTCAAGGTAGAG | 60 °C |