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. 2020 Jul 26;9(8):1780. doi: 10.3390/cells9081780

Table 1.

Primers used for PCR amplification.

Primer Name Sequence Anneling Temperature
mCherry NheI Forward GAGATCGCTAGCGGGCCCGCCACCATGGTGAGCAAGGGCGAGGAG 60.9 °C
mCherry XhoI CMV Reverse GAGATCCTCGAGCTACTTGTACAGCTCGTCCATG 60.9 °C
Promoter EF1ɑ Forward TCGGGTTTTTCGAACGGCTCCGGTGCCCGTCAG 60 °C
Promoter EF1ɑ Reverse ATGGTGGCGGGCCCGTCACGACACCTGAAATGGAAG 60 °C
Promoter PGK/UBC Forward GATCTCGACGGTATCGAAAGC 60 °C
Promoter PGK Reverse CCATGGTGGCGGGCCCGAATTCGATCTCGGATCCGAAAGCGAAGGAGCAAAGCTG 60 °C
Promoter UbC Reverse CCATGGTGGCGGGCCCGAATTCGATCTCGGATCCGTCTAACAAAAAAGCCAAAAAC 60 °C
AP3D1 Forward GGCATCCGTAACCACAAGGA 60 °C
AP3D1 Reverse TTGTCCTGCTTCAGCTCCTG 60 °C
CDK4 Forward GGCTGAAATTGGTGTCGGTG 60 °C
CDK4 Reverse CACGAACTGTGCTGATGGGA 60 °C
TERT Forward TGTACTTTGTCAAGGTGGATGTGA 60 °C
TERT Reverse GCTGGAGGTCTGTCAAGGTAGAG 60 °C