Table 3.

Summary of characterized immortalized human myogenic stem cell clones.

Patients	Clones	Population Doublings a	CD56 pos Cells (%)	Differentiation	Doubling Time (days) b
Control 1	B4	3.33	99.50	++	3.64
	D6	2.80	99.80	+++	2.86
	D52 long term	5.22	99.70	+++	2.3
		73.23	99.90	++
Control 2	E4	4.69	99.40	++	3.55
Control 3	A11	1.82	98.07	++	3.2
	A42 long term	3.87	98.20	++	2.15
		73.83	99.90	+++
CMD 1	D5	6.50	99.00	+++	2.51
	D10	8.73	98.90	++	5.43
	F22 long term	4.53	98.50	+++	3.15
		69.86	99.80	+++
	G4	5.15	98.91	+++	4.57
CMD 2	B7	4.74	99.80	+++	2.95
	G6	4.29	99.80	+++	3.39
	H3	5.80	99.70	+++	2.71
CMD 3	B6	4.42	96.00	++	3.5
	B12	2.00	97.30	+++	2.51
	F5	1.96	90.00	+++	9.1
CMD 4	D12	5.40	93.30	+++	5.76
	G42	2.51	91.00	+++	5.66
DMD 1	B12 long term	5.17	99.00	++	2.55
		66.39	93.40	++
	E92	3.39	99.00	++	6.62
	G8	3.48	97.20	+++	5.38
DMD 2	C4	2.07	86.00	+++	4.97
	C10	5.82	93.10	++	3.04
	G82	6.18	98.50	++	3.54
aDMD 3	A10 long term	3.90	99.30	++	3.36
		42.39	95.10	+
	B2	7.77	89.00	+++	3.07
DMD 4	B42	3.61	98.10	++	3.89
	H82 long term	5.39	99.80	++	2.84
		52.30	nd	+++
DMD 5	E82	4.32	98.00	++	5.93
	H2	2.56	100.00	++	5.51
DMD 6	E12	2.65	96.70	+++	3.62
	G11	6.45	93.20	+++	5.23
	H112	3.39	91.70	++	5.15
DMD 7	A3	5.80	93.70	+++	2.64
	B10	8.40	98.00	+++	4.19
	C12	6.80	99.30	+++	3.23
	E3 long term	15.96	99.00	++	2.27
		119.30	82.90	nd
DMD 8	A3 long term	5.72	99.20	++	1.91
		63.55	98.00	nd

In italic, long term culture; +, poor fusion; ++, small myotubes; +++, large myotubes; nd, non-determined. ^a Population doubling of the culture at the time of analysis of CD56 expression and of differentiation. ^b Doubling time along the culture from the initial seeding until the indicated population doubling.