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. 2020 Jul 28;12(8):2092. doi: 10.3390/cancers12082092

Figure 5.

Figure 5

MF-15 inhibits AR-FL and AR-V7 reporter gene activity. (A) AR negative PC-3 cells were transiently transfected with full-length AR (AR-FL) or AR-V7 alone and seeded into 96-well plates in RPMI + 2% FCS supplemented with 10 µM MF-15 alone or with increasing concentrations of MF-15 or 5 µM enzalutamide (Enza) and 0.2 nM of the synthetic androgen R1881. To exclude unspecific interpolation of MF-15 with the assay, luciferase activity was measured following treatment of transfected cells with 0.2 nM R1881 and 10 µM MF-15 for 2 h (R1881+MF-15 CO). (B) PC-3 cells were transiently transfected with AR-FL and AR-V7 at different ratios (95:5 and 80:20) and treated with 10 µM MF-15 alone or with 10 µM MF-15 or 2.5 µM enzalutamide and 0.2 nM R1881. Luciferase reporter gene activity was determined with Nano Glow Dual Assay (Promega) by measuring absorbance on a CYTATION multiplate reader instrument. Data represent the mean ±SEM from three independent experiments. Statistical comparisons to the mock control were expressed in the graph with a hash key (## p < 0.01, ### p < 0.001) and comparisons to R1881 with an asterisk (*) (* p< 0.05, *** p < 0.001).