Table 1.
Proteins or factors in mammals and Drosophila that were revealed as directly bound with the known domains of HP1a/HP1α.
| Protein or Cellular Component | Organism | Methodology | References | |
|---|---|---|---|---|
| CHD | ||||
| Methylated H3K9 | Drosophila | IF, FAITC, NMR | [51] | |
| H2Av | Drosophila | IF, tagIP, rPD, | [52] | |
| RpII215 | Drosophila | IP, WB, rPD | [7,53] | |
| Nuclear envelope | Mouse | IF, BA | [54] | |
| H3 | Mouse | IP, FW, rPD | [31,55] | |
| H1 | Mouse | rPD, FW | [55] | |
| Methylated H3K9 | Mouse | rPD | [56] | |
| Methylated H3K9 | Human | rPD, SPRA | [57] | |
| CTIP2 | Human | rPD, IP | [58,59] | |
| Methylated H1.4K26 | Human | BD, IP, rPD, IF | [31,37] | |
| DNMT1 | Human | rPD | [60] | |
| CSD | ||||
| Hip/HP4 | Drosophila | Y2H, tagIP, rPD, IF, tag-WB | [61,62] | |
| AF10/Alh | Drosophila | transPD | [42,63] | |
| Su(var)3-7 | Drosophila | Y2H, IP, IF, WB | [64,65] | |
| PIWI | Drosophila | Y2H, IP, IF, NMR, Y2H | [42,66,67] | |
| Kdm4A | Drosophila | transIP, tag-WB, MW, WB, fingerprinting, MS | [68,69,70] | |
| Ssrp | Drosophila | transPD, WB, IP, tandem affinity technology, tagIP, tag-WB | [53,71] | |
| Su(var)3-9 | Drosophila | transPD, At, ATC, WB, Y2H, IP, tag-WB, tagIP, fingerprinting, IF, MS | [53,68,70,72,73] | |
| Su(var)2-HP2 | Drosophila | IP, At, Y2H, NMR, FAITC, PP, tagIP, fingerprinting, co-sedimentation, molecular weight, molecular sieving, MS | [42,68,70,74,75] | |
| XNP/dATRX | Drosophila | transIP, transPD, MS, IF, WB | [68,76,77,78] | |
| HP6 | Drosophila | IP, WB, transPD, tag-WB | [79] | |
| egg | Drosophila | transIP, fingerprinting, rPD | [53,68] | |
| G9a | Drosophila | IP, WB, rPD | [53] | |
| ova | Drosophila | IP, Y2H | [80] | |
| HP1-BP84 | Mouse | Y2H | [81] | |
| TIF1α | Mouse | Y2H, rPD | [50,81,82] | |
| CAF-1 p150 | Mouse | Y2H, rPD, IF, GFC, NMR | [33,83] | |
| mSNF2β | Mouse | Y2H | [50] | |
| KAP1/TIFβ | Mouse | IP, rPD, IF, SPRA, GFC | [50,55,83] | |
| H4 | Mouse | In vitro cross-linking | [31] | |
| MeCP2 | Mouse | tagIP | [84] | |
| KAP1/TIFβ | Human | Y2H, IP, rPD, IF, GFC | [83,85] | |
| SP100 | Human | Y2H, rPD, transPD, IF | [86] | |
| Polycomb | Human | IP, rPD, IF | [33] | |
| ATRX | Human | Y2H, IF, rPD | [85,87] | |
| CAF-1 p150 | Human | rPD | [50] | |
| Ku70 | Human | Y2H, IP, rPD | [88] | |
| TAFII130 | Human | Y2H, exPD, transPD | [89] | |
| Ki-67 | Human | Y2H, exPD, IF, ChIP | [90] | |
| BRG1 | Human | IP, rPD, TransPD, IF | [91] | |
| SUV39H1 | Human | rPD, Y2H | [49] | |
| NIPBL/hScc2 | Human | rPD | [85,92] | |
| HP1-BP74 | Human | rPD | [85] | |
| LBR (Lamin B receptor) | Human | rPD, Y2H, IP | [85,93] | |
| Sgo1 | Human | Y2H, MS. IP | [92,94] | |
| POGZ | Human | Y2H, MS | [92] | |
| BARD1 | Human | tragIP, transPD | [95] | |
| KDM2A | Human | IP, transPD, IF | [96] | |
| LRIF1 | Human | IP, transPD | [97] | |
| Haspin | Human | tragIP, rPD | [98] | |
| MacroH2A1.2 | Human | IP, transPD | [99] | |
| Hinge | ||||
| HP-BP74 H1-like | Mouse | Y2H, FW, rPD | [55,81] | |
| MITR, HDAC4/5 | Mouse | IP, rPD | [100] | |
| Combination of Domains | ||||
| ORC1-6 | Drosophila | tagIP | CHD, CSD | [30] |
| Mcm10 | Drosophila | proximity ligation assay, IF, IP, WB, transPD, tag-WB, Y2H | CHD, CSD | [101,102] |
| SuUR | Drosophila | Y2H, rPD, transPD, WB, IP, MS, tagIP, fingerprinting | Hin + CSD | [68,93] |
| Caf1-180 | Drosophila | transPD, tag-WB, WB, transIP, fingerprinting, IP | Hin + CSD | [68,103] |
| Cav/HOAP | Drosophila | tagIP, IF, IP, exPD | Hin + CSD | [79,104,105,106,107] |
| Parp-2 | Mouse | rPD | Hin + CSD | [108] |
| TIf1β | Mouse | rPD | Hin + CSD | [108] |
| ARFL5 | Human | Y2H, rPD | CHD + CSD | [109] |
| INCENP | Human | Y2H, tranPD | Hin + CSD | [94,110] |
Methodology: BA, binding assays; ChIP, chromatin immunoprecipitation; IP, co-immunoprecipitation using extract; exPD, pull-down assay using extracts; FAITC, fluorescence anisotropy, isothermal titration calorimetry; FW, far-western analysis; IF, immunofluorescence co-localization; rPD, pull-down assay using recombinant proteins; tragIP, immunoprecipitation with in-vitro translated protein; transPD, pull-down assay using in-vitro translated protein; SPRA, surface plasmon resonance analysis; Y2H, yeast two-hybrid assay; WB, western blot; NMR, nuclear magnetic resonance; PP, predetermined participant; tag-WB, western blot assay performed when specific antibodies for the protein of interest are not available; At, autoradiography; fingerprinting, peptide mass fingerprinting; MS, identification by mass spectrometry; BiFC, bimolecular fluorescence complementation.