Figure 3.

Select cytokine gene activation in B cells upon TMEV infection. (A) Isolated splenic B cells were infected with two different stocks of TMEV (MOI = 2) for 48 h, and then the levels of IFN-α4, IFN-β, and IL-6 were determined using RT-PCR. (B) The levels of an activation marker (CD69) of CD4 and CD19 in TMEV-infected or uninfected IFNα/βR-deficient and control 129S2 mice were determined using flow cytometry. The presented data are representative of three independent experiments (n = 5).