Table 2.

Analysis of isothiocyanates in broccoli sprouts and microgreens.

No.	Compounds	Molecular Formula	Germination Time	Characterization Method	Ref.
1	Butyronitrile	C4H7N	3–7 days	GC–MS	[67]
2	Allyl isothiocyanate	C4H5NS	4 days	GC–MS	[66]
3	2-Methyl-2-nitropropane	C4H9NO2	3–7 days	GC–MS	[67]
4	4-(Methylthio)-butanenitrile	C5H9NS	5 days	GC–MS	[42]
5	Butyl isothiocyanate	C5H9NS	4–9 days	GC–MS	[42,44,66,67]
6	Isobutyl isothiocyanate	C5H9NS	4 days	GC–MS	[19]
7	Iberin	C5H9NOS2	4–8 days	HPLC-DAD, UHPLC-MS/MS, GC–MS	[19,53,66]
8	4-Isothiocyanato-1-butene	C6H9NS2	4–9 days	GC–MS	[42,44,66]
9	3-Methylbutyl isothiocyanate	C6H11NS	4 days	GC–MS	[66]
10	Isoamyl methyl sulfoxide	C6H14OS	3–7 days	GC–MS	[67]
11	Erucin	C6H11NS2	3–9 days	UHPLC-MS/MS, GC–MS	[19,44,56,61,67]
12	Sulforaphene	C6H9NOS2	8 days	UHPLC-MS/MS	[19]
13	Sulforaphane	C6H11NOS2	2–12 days	HPLC-UV, HPLC-DAD, HPLC-DAD-MS, HPLC-MS/MS, UHPLC-MS/MS, GC–MS, GC−FID	[19,22,42,44,46,48,53,56,57,59,60,61,62,63,64,66,67,68,69,70,71,72]
14	Indole-3-carbinol	C9H9NO	8 days	UHPLC-MS/MS	[19]
15	Indole-3-carboxylic acid	C9H7NO2	NS	HPLC-DAD-MS	[46]
16	Indole-3-acetic acid	C10H9NO2	NS	HPLC-DAD-MS	[46]
17	1-Methoxyindole-3-carbaldehyde	C10H9NO2	NS	HPLC-DAD-MS	[46]
	Total isothiocyanates		8 days	UV/Vis, UHPLC-MS	[19,20,73]

UV/Vis, ultraviolet–visible spectrophotometry; GC–MS, gas chromatography combined with mass spectrometry; GC−FID, GC with a flame ionization detector.