Figure 2.

Dysregulated miRNAs in uninfected M cells compared to uninfected Caco-2-cl1 cells. A total of 10⁷ LF82 bacteria were added into the apical compartment of M cells or the Caco-2-cl1 monolayers. At 4 h post-infection, the monolayers were collected, washed with cold PBS, and total RNA was extracted and used for miRNA array analysis. The graph shows the relative expression of the miRNAs that were dysregulated in uninfected M cells versus uninfected Caco-2-cl1 cells, presented as fold change (normalized to expression levels of miRNAs in uninfected Caco-2-cl1 cells, defined as 1).