Figure 3.

Comparison of sevanol (s706) and the Phe-Arg-Arg-Phe-amide peptide (FRRFa) effects on the ASIC1a current. (A) Representative traces of pH 5.5-evoked currents of ASIC1a alone (black trace) and of ASIC1a with a 15 s pre-incubation of 300 µM s706 (red trace), 200 µM FRRFa (green trace), or a mixture of 300 µM s706 and 200 µM FRRFa (blue trace). (B) Dose–response ASIC1a inhibitory curves for s706 alone (red line) and for s706 in the presence of 200 µM FRRFa (blue line), determined for integral currents. Data were fitted by the logistic equation. Data are presented as mean ± SEM (n = 5).