Table 2.

The sequences and annealing temperature of endoplasmic reticulum stress-associated primers.

Target mRNA	Annealing Temperature (°C)	Forward Primer Sequence (5′-3′)	Reverse Primer Sequence (5′-3′)
GRP78	59.3	TGACATTGAAGACTTCAAAGCT	CTGCTGTATCCTCTTCACCAGT
Total XBP1	59.3	GGCATCCTGGCTTGCCTCCA	GCCCCCTCAGCAGGTGTTCC
ERDJ4	59.3	TCGGCATCAGAGCGCCAAATCA	ACCACTAGTAAAAGCACTGTGTCCAAG
CHOP	59.3	GGAGCATCAGTCCCCCACTT	TGTGGGATTGAGGGTCACATC
GADD34	59.3	CCCAGAAACCCCTACTCATGATC	GCCCAGACAGCCAGGAAAT

Abbreviations: GRP78, glucose-regulated protein 78 kDa; total XBP1, total X-box-binding protein 1; ERDJ4, ER-DnaJ-like 4; CHOP, cholesterol oxidase-peroxidase C/EBP homologous protein; and GADD34, growth arrest and DNA damage-inducible gene 34.