Figure 6.

Using conventional electron microscopy with 70 nm thick sections, the probability of observing a dense granule is low. An alternative is the reconstruction of serial sections. The rapid, more, or less automated method of focused ion beam-scanning electron microscopy (FIB-SEM) makes it possible to observe the spatial distribution of α- (yellow) and dense-granules (black) in a platelet. (A) data from Eckly et al. published in [76] (part of Figure 6B, image obtained from the Haematologica Journal website http://www.haematologica.org). A stereo image of an entire platelet on formvar can be obtained from two tilt images taken at ±7°. (B) Anaglyph reconstructed with ImageJ (V1.8 NIH, USA) Two Shot Anaglyph software (V2.9.5, Sandy Knoll Software, USA); the stereo effect is visible with red-cyan glasses.