Figure 2.

Anti-Gly-HSA-Abs were detected by direct binding ELISA form serum samples (1:100 diluted) of all the subjects in different groups. Groups were divided based on age and smoking habits; group I (21–40), I-S (21–40), II (41–60), II-S (41–60), III (61–80), III-S (61–80), IV (>80) and IV-S (>80). ‘S’ in the group represent subjects who are smokers. Data presented as mean ± SD. t test was adopted for the comparison between the two groups and significance is defined as ** p  <  0.01, *** p  <  0.001. The comparison between group IV-S and IV showed p < 0.001. The comparison between III-S and III showed p < 0.01. The comparison between IV and III showed p < 0.01.