Table 1.
Chemical and structural characterizations of native human serum albumin (N-has) and glycation of human serum albumin (Gly-has).
| Parameters | N-HSA | Gly-HSA |
|---|---|---|
| Ketoamine (mol/mol of HSA) | 0.25 ± 0.1 | 6.4 ± 0.3 ** |
| Carbonyl content (mol/mg of HSA) | 0.1 ± 0.02 | 3.11 ± 0.4 *** |
| Pentosidine Fluorescence (AU) Exc. at 275 nm |
5.2 ± 0.7 | 247.5 ± 8.7 *** |
| Serum Pentosidine (µg/mL) ELISA method |
0.0092 ± 0.0004 | 0.0863 ± 0.012 ** |
N-HSA was considered as standard in all the estimations. Each value represents arithmetic mean ± SD of three independent assays. Ext. represents excitation emission wavelength. t test was adopted for the comparison between the two groups and significance is defined as ** p < 0.01, *** p < 0.001.