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. 2020 Aug 15;12(8):2300. doi: 10.3390/cancers12082300

Figure 3.

Figure 3

Knockdown of ITGA2 in PC-3 cells suppresses the effect of exosomes on recipient C4-2B and LNCaP cells. (A). PC-3 cells were transduced with shRNA specific to ITGA2 in addition to scrambled control. Immunoblot (IB) analysis was performed to examine the expression of ITGA2 in total cell and exosomes lysates. (B). C4-2B and LNCaP cells were incubated with 20 µg/mL exosomes derived from ITGA2 knockdown PC-3 cells. (C) C4-2B cells were incubated with 10 and 20 µg/mL exosomes derived from ITGA2 knockdown PC-3 cells for 24 h. RNA was extracted and real-time PCR was performed to determine the expression of ITGA2 relative to gapdh. (D,E) C4-2B and LNCaP cells were incubated with exosomes isolated from the integrin knockdown PC-3 cells and cell proliferation and migration assays were assessed. Data considered significant at p < 0.05. The experiment was repeated twice.