Figure 5.

Ectopic expression of ITGA2 promotes aggressive phenotypes in PCa cells. C4-2B and E006AA cells were transfected with 2.5 µg plasmid carrying ITGA2 or pcDNA3.1 as a control plasmid for 96 h. The expression of ITGA2, E-cadherin (E-Cad), vimentin (Vim), and c-Myc, and the activity of FAK and ERK1/2 were evaluated in these cells (A,F). Cell proliferation (B,G) and clonogenic (C,H) assays in the ITGA2-transfected cells were performed. Transwell migration (D, I) and invasion (E,J) assays were also conducted over a period of 18 to 48 h. * depicts significance at p < 0.05. Each experiment was performed in triplicate and independently repeated three times.